Mechanical ventilation enhances Acinetobacter baumannii-induced lung injury through JNK pathways.

ABSTRACT:

Background

Patients in intensive care units (ICUs) often received broad-spectrum antibiotic treatment and Acinetobacter baumannii (A.b.) and Pseudomonas aeruginosa (P.a.) were the most common pathogens causing ventilator-associated pneumonia (VAP). This study aimed to examine the effects and mechanism of mechanical ventilation (MV) on A.b.-induced lung injury and the involvement of alveolar macrophages (AMs).

Methods

C57BL/6 wild-type (WT) and c-Jun N-terminal kinase knockout (JNK1^-/-) mice received MV for 3 h at 2 days after nasal instillation of A.b., P.a. (1 × 10⁶ colony-forming unit, CFU), or normal saline.

Results

Intranasal instillation of 10⁶ CFU A.b. in C57BL/6 mice induced a significant increase in total cells and protein leve

SUBMITTER: Tsay TB 

PROVIDER: S-EPMC8140754 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature