Correlation of Cry1Ac mRNA and protein abundance in transgenic Gossypium hirsutum plant.

ABSTRACT: Transcription and translation in eukaryotes are distinct processes of the molecular cascade leading to protein production from genetic material. However, establishing correlation between mRNA expression and protein abundance, the end results of the two processes of central dogma, remains a challenge. For transgenic plants, such correlation between mRNA and protein expression serves as a guide to design the transgene, in particular the choices of promoter and codon usage to ensure stable expression of the target protein in relevant tissues under various stress conditions. To elucidate level of mRNA-protein correlation in a commercial transgenic cotton plant Gossypium hirsutum, Bollgard II^® (MON15985), we present the results of Cry1Ac protein expression correlating with cor

SUBMITTER: Smitha PK 

PROVIDER: S-EPMC8141090 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature