A comparative analysis of the intrauterine transcriptome in fertile and subfertile mares using cytobrush sampling.

ABSTRACT:

Background

Subfertility is a major problem in modern horse breeding. Especially, mares without clinical signs of reproductive diseases, without known uterine pathogens and no evidence of inflammation but not becoming pregnant after several breeding attempts are challenging for veterinarians. To obtain new insights into the cause of these fertility problems and aiming at improving diagnosis of subfertile mares, a comparative analysis of the intrauterine transcriptome in subfertile and fertile mares was performed. Uterine cytobrush samples were collected during estrus from 57 mares without clinical signs of uterine diseases. RNA was extracted from the cytobrush samples and samples from 11 selected subfertile and 11 fertile mares were used for Illumina RNA-sequencing.

Results

T

SUBMITTER: Weber KS 

PROVIDER: S-EPMC8141133 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature