MiR-501 promotes tumor proliferation and metastasis by targeting HOXD10 in endometrial cancer.

ABSTRACT:

Background

Several studies have shown the crucial role of miR-501 in regulating cellular pathology in various cancers. However, the function and expression of miR-501 in endometrial cancer (EC) remain obscure.

Methods

The expression of miR-501 was determined using quantitative real-time PCR. MTT assay, colony formation assay and cell cycle analysis were used to evaluate the proliferation ability. Migration and invasion were assessed using transwell assay. Tumor formation in nude mice was used to observe the effects of miR-501 on cell proliferation and migration in vivo. Luciferase assay, quantitative real-time PCR and western blot were applied to determine that HOXD10 was the target gene of miR-501.

Results

In this study, we observed significantly up-regulated express

SUBMITTER: Sun X 

PROVIDER: S-EPMC8141179 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature