Quantitative proteomic analysis of aberrant expressed lysine acetylation in gastrointestinal stromal tumors.

ABSTRACT:

Background

Gastrointestinal stromal tumor (GIST) is a common digestive tract tumor with high rate of metastasis and recurrence. Currently, we understand the genome, transcriptome and proteome in GIST. However, posttranscriptional modification features in GIST remain unclear. In the present study, we aimed to construct a complete profile of acetylome in GIST.

Methods

Five common protein modifications, including acetylation, succinylation, crotonylation, 2-hydroxyisobutyrylation, and malonylation were tested among GIST subgroups and significantly differentially- expressed lysine acetylation was found. The acetylated peptides labeled with Tandem Mass Tag (TMT)under high sensitive mass spectrometry, and some proteins with acetylation sites were identified. Subsequently, these pr

SUBMITTER: Wang B 

PROVIDER: S-EPMC8141230 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature