Circadian Clock Disruption Suppresses PDL1⁺ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.

ABSTRACT:

Background & aims

The circadian clock is crucial for physiological homeostasis including gut homeostasis. Disorder of the circadian clock may contribute to many diseases including inflammatory bowel disease (IBD). However, the role and the mechanisms of circadian clock involvement in IBD still are unclear.

Methods

Disorder of the circadian clock including chronic social jet lag and circadian clock gene deficiency mice (Bmal1^-/-, and Per1^-/-Per2^-/-) were established. Dextran sulfate sodium (DSS) and/or azoxymethane were used to induce mouse models of colitis and its associated colorectal cancer. Flow cytometry, immunohistochemistry, immunofluorescence, Western blot, and reverse-transcription quantitative polymerase chain reaction were used to

SUBMITTER: Liu JL 

PROVIDER: S-EPMC8141473 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature