The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine Sponge Geodia barretti.

ABSTRACT: Geodia barretti is a deep-sea marine sponge common in the north Atlantic and waters outside of Norway and Sweden. The sampling and subsequent treatment as well as storage of sponges for metabolomics analyses can be performed in different ways, the most commonly used being freezing (directly upon collection or later) or by storage in solvent, commonly ethanol, followed by freeze-drying. In this study we therefore investigated different sampling protocols and their effects on the detected metabolite profiles in liquid chromatography-mass spectrometry (LC-MS) using an untargeted metabolomics approach. Sponges (G. barretti) were collected outside the Swedish west coast and pieces from three sponge specimens were either flash frozen in liquid nitrogen, frozen later after the colle

SUBMITTER: Erngren I 

PROVIDER: S-EPMC8141568 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature