Exosomally derived Y RNA fragment alleviates hypertrophic cardiomyopathy in transgenic mice.

ABSTRACT: Cardiosphere-derived cell exosomes (CDC_exo) and YF1, a CDC_exo-derived non-coding RNA, elicit therapeutic bioactivity in models of myocardial infarction and hypertensive hypertrophy. Here we tested the hypothesis that YF1, a 56-nucleotide Y RNA fragment, could alleviate cardiomyocyte hypertrophy, inflammation, and fibrosis associated with hypertrophic cardiomyopathy (HCM) in transgenic mice harboring a clinically relevant mutation in cardiac troponin I (cTnI^Gly146). By quantitative PCR, YF1 was detectable in bone marrow, spleen, liver, and heart 30 min after intravenous (i.v.) infusion. For efficacy studies, mice were randomly allocated to receive i.v. YF1 or vehicle, monitored for ambulatory and cardiac function, and sacrificed at 4 weeks. YF1 (but not vehi

SUBMITTER: Huang F 

PROVIDER: S-EPMC8141670 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature