How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?

ABSTRACT:

Objectives

The SARS-CoV-2 pandemic has created an unprecedented need for rapid large-scale diagnostic testing to prompt clinical and public health interventions. Currently, several quantitative reverse-transcription polymerase chain reaction (RT-qPCR) assays recommended by the World Health Organization are being used by clinical and public health laboratories and typically target regions of the RNA-dependent RNA polymerase (RdRp), envelope (E) and nucleocapsid (N) coding region. However, it is currently unclear if results from different tests are comparable. This study aimed to clarify the clinical performances of the primer/probe sets designed by US CDC and Charité/Berlin to help clinical laboratories in assay selection for SARS-CoV-2 routine detection.

Methods

We compared

SUBMITTER: Anantharajah A 

PROVIDER: S-EPMC8141720 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature