A new mouse SNP genotyping assay for speed congenics: combining flexibility, affordability, and power.

ABSTRACT:

Background

Speed congenics is an important tool for creating congenic mice to investigate gene functions, but current SNP genotyping methods for speed congenics are expensive. These methods usually rely on chip or array technologies, and a different assay must be developed for each backcross strain combination. "Next generation" high throughput DNA sequencing technologies have the potential to decrease cost and increase flexibility and power of speed congenics, but thus far have not been utilized for this purpose.

Results

We took advantage of the power of high throughput sequencing technologies to develop a cost-effective, high-density SNP genotyping assay that can be used across many combinations of backcross strains. The assay surveys 1640 genome-wide SNPs known to be poly

SUBMITTER: Andrews KR 

PROVIDER: S-EPMC8142480 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature