Diverse cellular morphologies during lumen maturation in Anopheles gambiae larval salivary glands.

ABSTRACT: Mosquitoes are the greatest animal threat to human health, causing hundreds of millions of infections and around 1 million deaths each year. All mosquito-borne pathogens must traverse the salivary glands (SGs) to be transmitted to the next host, making this organ an ideal target for interventions. The adult SG develops from precursor cells located in the larval SG duct bud. Characterization of the larval SG has been limited. We sought to better understand larval SG architecture, secretion and gene expression. We developed an optimized method for larval SG staining and surveyed hundreds of larval stage 4 (L4) SGs using fluorescence confocal microscopy. Remarkable variation in SG cell and chromatin organization differed among individuals and across the L4 stage. Lumen formation occurred duri

SUBMITTER: Chiu M 

PROVIDER: S-EPMC8142555 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature