An Optimized Real-Time qPCR Method for the Effective Detection of Human Malaria Infections.

ABSTRACT: Polymerase chain reaction, although an expensive method for the detection of human Plasmodium spp., is still considered the finest for the diagnosis of malaria. The conventional diagnostic PCR is an inexpensive process but consumes a lot of time, reagents and lacks sensitivity. On the other hand, real-time PCR assays currently being used are mostly probe-based expensive methods and sometimes not feasible to detect all the species in a single amplification reaction condition. Here we have established a real-time PCR method that is time and cost effective with a single protocol to detect and distinguish five human Plasmodium species using the existing primers efficiently. The primers used here are being used in the conventional method and the sensitivity as well as specificity of this method

SUBMITTER: Sazed SA 

PROVIDER: S-EPMC8142979 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature