Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana.

ABSTRACT: Cells of many organisms and organs can withstand an (almost) total water loss (anhydrobiosis). Sugars play an essential role in desiccation tolerance due to their glass formation ability during dehydration. In addition, intrinsically disordered LEA proteins contribute to cellular survival under such conditions. One possible mechanism of LEA protein function is the stabilization of sugar glasses. However, little is known about the underlying mechanisms. Here we used FTIR spectroscopy to investigate sucrose (Suc) glass stability dried from water or from two buffer components in the presence of four recombinant LEA and globular reference proteins. Buffer ions influenced the strength of the Suc glass in the order Suc < Suc/Tris < Suc/NaP. LEA proteins strengthened the sugar H-bonded network an

SUBMITTER: Hincha DK 

PROVIDER: S-EPMC8143093 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature