Towards the Elucidation of Assimilative nasABC Operon Transcriptional Regulation in Haloferax mediterranei.

ABSTRACT: The assimilatory pathway of the nitrogen cycle in the haloarchaeon Haloferax mediterranei has been well described and characterized in previous studies. However, the regulatory mechanisms involved in the gene expression of this pathway remain unknown in haloarchaea. This work focuses on elucidating the regulation at the transcriptional level of the assimilative nasABC operon (HFX_2002 to HFX_2004) through different approaches. Characterization of its promoter region using β-galactosidase as a reporter gene and site-directed mutagenesis has allowed us to identify possible candidate binding regions for a transcriptional factor. The identification of a potential transcriptional regulator related to nitrogen metabolism has become a real challenge due to the lack of information on haloarchaea.

SUBMITTER: Pastor-Soler S 

PROVIDER: S-EPMC8143581 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature