Blocking core fucosylation of epidermal growth factor (EGF) receptor prevents peritoneal fibrosis progression.

ABSTRACT:

Objective

Peritoneal fibrosis (PF) ultimately causes ultrafiltration failure and peritoneal dialysis (PD) termination, but there are few effective therapies for it. Core fucosylation, which is catalyzed by α1,6-fucosyltransferase (Fut8) in mammals, may play a crucial role in PF development. This study aims to assess the effects of inhibiting core fucosylation of epidermal growth factor (EGF) receptor on PF rats.

Methods

PF rats (established by 4.25% glucose dialysate) were treated with either an adenovirus-Fut8 short hairpin RNA (Fut8shRNA) or adenovirus-control. Masson's staining and net ultrafiltration were performed at week six. Fut8 level and core fucosylation of EGF receptor and collagen I in the peritoneal membrane were assessed, and EGF signaling was detected, includi

SUBMITTER: Yu C 

PROVIDER: S-EPMC8143636 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature