An Interaction Network of RNA-Binding Proteins Involved in Drosophila Oogenesis.

ABSTRACT: During Drosophila oogenesis, the localization and translational regulation of maternal transcripts relies on RNA-binding proteins (RBPs). Many of these RBPs localize several mRNAs and may have additional direct interaction partners to regulate their functions. Using immunoprecipitation from whole Drosophila ovaries coupled to mass spectrometry, we examined protein-protein associations of 6 GFP-tagged RBPs expressed at physiological levels. Analysis of the interaction network and further validation in human cells allowed us to identify 26 previously unknown associations, besides recovering several well characterized interactions. We identified interactions between RBPs and several splicing factors, providing links between nuclear and cytoplasmic events of mRNA regulation. Addi

SUBMITTER: Bansal P 

PROVIDER: S-EPMC8143644 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature