Adapting a Low-Cost and Open-Source Commercial Pipetting Robot for Nanoliter Liquid Handling.

ABSTRACT: Low-volume liquid handling capabilities in bioanalytical workflows can dramatically improve sample processing efficiency and reduce reagent costs, yet many commercial nanoliter liquid handlers cost tens of thousands of dollars or more. We have successfully adapted a low-cost and open-source commercial pipetting robot, the Opentrons OT-1, to accurately aspirate and dispense nanoliter volumes. Based on fluorescence measurements, the modified OT-1 was able to reproducibly transfer 50 nL of water with less than 3% measurement error and 5% coefficient of variation (CV). For 15 nL transfers, the volume measurements indicated less than 4% error and 4% CV. We applied this platform to the preparation of low-nanogram proteomic samples for liquid chromatography-mass spectrometry analysis, demonstrati

SUBMITTER: Councill EEAW 

PROVIDER: S-EPMC8143861 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature