Sevoflurane Suppresses Colon Cancer Cell Malignancy by Regulating circ-PI4KA.

ABSTRACT:

Purpose

To explore the effect of SEV on colon cancer cells through circ-PI4KA.

Methods

The RNA level of circular RNA_0062389, microRNA-331-3p and LIM and SH3 protein 1 was determined by quantitative real-time polymerase chain reaction. Protein expression was detected by Western blot. Cell proliferation was investigated by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide, cell colony formation and 5-ethynyl-29-deoxyuridine assays. Cell apoptosis was demonstrated using Annexin V-fluorescein isothiocyanate/propidium iodide double staining assay. Cell migration and invasion were detected by transwell assay. The target relationship between miR-331-3p and circ-PI4KA or LASP1 was predicted by starBase v2.0 online database, and identified by a dual-luciferase reporter

SUBMITTER: Sun S 

PROVIDER: S-EPMC8144176 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature