Predicting MHC I restricted T cell epitopes in mice with NAP-CNB, a novel online tool.

ABSTRACT: Lack of a dedicated integrated pipeline for neoantigen discovery in mice hinders cancer immunotherapy research. Novel sequential approaches through recurrent neural networks can improve the accuracy of T-cell epitope binding affinity predictions in mice, and a simplified variant selection process can reduce operational requirements. We have developed a web server tool (NAP-CNB) for a full and automatic pipeline based on recurrent neural networks, to predict putative neoantigens from tumoral RNA sequencing reads. The developed software can estimate H-2 peptide ligands, with an AUC comparable or superior to state-of-the-art methods, directly from tumor samples. As a proof-of-concept, we used the B16 melanoma model to test the system's predictive capabilities, and we report its putative neoan

SUBMITTER: Wert-Carvajal C 

PROVIDER: S-EPMC8144223 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature