Detection of urinary microRNA biomarkers using diazo sulfonamide-modified screen printed carbon electrodes.

ABSTRACT: This paper describes a straightforward electrochemical method for rapid and robust urinary microRNA (miRNA) quantification using disposable biosensors that can discriminate between urine from diabetic kidney disease (DKD) patients and control subjects. Aberrant miRNA expression has been observed in several major human disorders, and we have identified a urinary miRNA signature for DKD. MiRNAs therefore have considerable promise as disease biomarkers, and techniques to quantify these transcripts from clinical samples have significant clinical and commercial potential. Current RT-qPCR-based methods require technical expertise, and more straightforward methods such as electrochemical detection offer attractive alternatives. We describe a method to detect urinary miRNAs using diazo sulfonamide

SUBMITTER: Smith DA 

PROVIDER: S-EPMC8144888 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature