Low Collision Energy Fragmentation in Structure-Specific Glycoproteomics Analysis.

ABSTRACT: Glycosylation is a major post-translational modification of proteins that regulates many biological processes including protein folding, structure stability, receptor activation, and immune responses. The glycans attached to proteins represent an important determinant of the protein interaction-specificity and maintain the 3D structure of proteins. Mass spectrometry (MS) is one of the most efficient tools used in the current studies of glycoproteins and structure of their glycoforms. Collision energy (CE) is a crucial instrument parameter that can be exploited to improve structural resolution because different linkages of glycan units show different stabilities under CID/HCD fragmentation. Here we report the utility of CE modulation for qualitative and quantitative analysis of site- and st

SUBMITTER: Sanda M 

PROVIDER: S-EPMC8145010 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature