Methylome and transcriptome signature of bronchoalveolar cells from multiple sclerosis patients in relation to smoking.

ABSTRACT:

Background

Despite compelling evidence that cigarette smoking impacts the risk of developing multiple sclerosis (MS), little is known about smoking-associated changes in the primary exposed lung cells of patients.

Objectives

We aimed to examine molecular changes occurring in bronchoalveolar lavage (BAL) cells from MS patients in relation to smoking and in comparison to healthy controls (HCs).

Methods

We profiled DNA methylation in BAL cells from female MS (n = 17) and HC (n = 22) individuals, using Illumina Infinium EPIC and performed RNA-sequencing in non-smokers.

Results

The most prominent changes were found in relation to smoking, with 1376 CpG sites (adjusted P < 0.05) differing between MS smokers and non-smokers. Approximately 30% of t

SUBMITTER: Ringh MV 

PROVIDER: S-EPMC8145441 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature