Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures.

ABSTRACT: Lentiviral vectors (LVs) are a powerful tool for gene and cell therapy and human embryonic kidney cells (HEK293) have been extensively used as a platform for production of these vectors. Like most cells and cellular tissues, HEK293 cells release extracellular vesicles (EVs). EVs released by cells share similar size, biophysical characteristics and even a biogenesis pathway with cell-produced enveloped viruses, making it a challenge to efficiently separate EVs from LVs. Thus, EVs co-purified with LVs during downstream processing, are considered "impurities" in the context of gene and cell therapy. A greater understanding of EVs co-purifying with LVs is needed to enable improved downstream processing. To that end, EVs from an inducible lentivirus producing cell line were studied under two co

SUBMITTER: Do Minh A 

PROVIDER: S-EPMC8145507 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature