Optimization of hemolysis, icterus and lipemia interference thresholds for 35 clinical chemistry assays.

ABSTRACT:

Objectives

Interference of chemistry assays by hemolysis, icterus and lipemia (HIL) was investigated on the Abbott Alinity c system. We sought to empirically establish optimized HIL index thresholds for the purposes of reporting HIL interference in a hospital laboratory and advising clinicians on the interpretation of laboratory results in the presence of hemolysis, icterus or lipemia.

Methods

HIL index values measured by spectrophotometry were compared with concentrations of hemoglobin, bilirubin and Intralipid. HIL interference of 35 Abbott Alinity chemistry assays was subsequently investigated by pairwise comparison of test results in pooled serum or plasma with those in test preparations spiked with hemolysate, bilirubin or Intralipid. Data generated from the interferenc

SUBMITTER: Ho CKM 

PROVIDER: S-EPMC8145753 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature