A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins.

ABSTRACT:

Objectives

To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.

Design and methods

304 sera tested by gel electrophoresis during 8 routine laboratory days were concurrently tested by capillary electrophoresis. Gels were read by an experienced medical technologist while capillary profiles by a Sebia representative and the same technologist. Most sera (214 of 304, 70%) were also analyzed by immunofixation electrophoresis, used here as the gold standard to calculate sensitivity and specificity of the gel and capillary systems.

Results

Gel and capillary estimated the concentration of albumin, gamma region, and M-spikes nearly perfectly, and that of beta, alpha-2, and alpha-1 reg

SUBMITTER: Howard BM 

PROVIDER: S-EPMC8145771 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature