Post-Translational Modification Networks of Contractile and Cellular Stress Response Proteins in Bladder Ischemia.

ABSTRACT: Molecular mechanisms underlying bladder dysfunction in ischemia, particularly at the protein and protein modification levels and downstream pathways, remain largely unknown. Here we describe a comparison of protein sequence variations in the ischemic and normal bladder tissues by measuring the mass differences of the coding amino acids and actual residues crossing the proteome. A large number of nonzero delta masses (11,056) were detected, spanning over 1295 protein residues. Clustering analysis identified 12 delta mass clusters that were significantly dysregulated, involving 30 upregulated (R² > 0.5, ratio > 2, p < 0.05) and 33 downregulated (R² > 0.5, ratio < -2, p < 0.05) proteins in bladder ischemia. These protein residues had different mass weights

SUBMITTER: Yang JH 

PROVIDER: S-EPMC8145895 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature