Diurnal Variation of Plasma Extracellular Vesicle Is Disrupted in People Living with HIV.

ABSTRACT: Several types of extracellular vesicles (EVs) secreted by various immune and non-immune cells are present in the human plasma. We previously demonstrated that EV abundance and microRNA content change in pathological conditions, such as HIV infection. Here, we investigated daily variations of large and small EVs, in terms of abundance and microRNA contents in people living with HIV (PLWH) receiving antiretroviral therapy (HIV+ART) and uninfected controls (HIV-). Venous blood samples from n = 10 HIV+ART and n = 10 HIV- participants were collected at 10:00 and 22:00 the same day. Large and small plasma EVs were purified, counted, and the mature miRNAs miR-29a, miR-29b, miR-92, miR-155, and miR-223 copies were measured by RT-PCR. Large EVs were significantly bigger in the plasma collected at 1

SUBMITTER: Bazie WW 

PROVIDER: S-EPMC8145918 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature