Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics.

ABSTRACT: We have developed a robust workflow to measure high-resolution fluxotypes (metabolic flux phenotypes) for large strain libraries under fully controlled growth conditions. This was achieved by optimizing and automating the whole high-throughput fluxomics process and integrating all relevant software tools. This workflow allowed us to obtain highly detailed maps of carbon fluxes in the central carbon metabolism in a fully automated manner. It was applied to investigate the glucose fluxotypes of 180 Escherichia coli strains deleted for y-genes. Since the products of these y-genes potentially play a role in a variety of metabolic processes, the experiments were designed to be agnostic as to their potential metabolic impact. The obtained data highlight the robustness of E. coli's

SUBMITTER: Berges C 

PROVIDER: S-EPMC8145925 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature