Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars.

ABSTRACT: Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition

SUBMITTER: Spedalieri C 

PROVIDER: S-EPMC8145934 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature