Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4⁺ T Cell Responsiveness.

ABSTRACT: Intermittent fasting and fasting mimetic diets ameliorate inflammation. Similarly, serum extracted from fasted healthy and asthmatic subjects' blunt inflammation in vitro, implicating serum components in this immunomodulation. To identify the proteins orchestrating these effects, SOMAScan technology was employed to evaluate serum protein levels in healthy subjects following an overnight, 24-h fast and 3 h after refeeding. Partial least square discriminant analysis identified several serum proteins as potential candidates to confer feeding status immunomodulation. The characterization of recombinant IGFBP1 (elevated following 24 h of fasting) and PYY (elevated following refeeding) in primary human CD4⁺ T cells found that they blunted and induced immune activation, respectively. F

SUBMITTER: Han K 

PROVIDER: S-EPMC8146063 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature