Production of a Human Histamine Receptor for NMR Spectroscopy in Aqueous Solutions.

ABSTRACT: G protein-coupled receptors (GPCRs) bind a broad array of extracellular molecules and transmit intracellular signals that initiate physiological responses. The signal transduction functions of GPCRs are inherently related to their structural plasticity, which can be experimentally observed by spectroscopic techniques. Nuclear magnetic resonance (NMR) spectroscopy in particular is an especially advantageous method to study the dynamic behavior of GPCRs. The success of NMR studies critically relies on the production of functional GPCRs containing stable-isotope labeled probes, which remains a challenging endeavor for most human GPCRs. We report a protocol for the production of the human histamine H1 receptor (H1R) in the methylotrophic yeast Pichia pastoris for NMR experiments. Systematic ev

SUBMITTER: Mulry E 

PROVIDER: S-EPMC8146376 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature