SmFISH in chips: a microfluidic-based pipeline to quantify in situ gene expression in whole organisms.

ABSTRACT: Gene expression and genetic regulatory networks in multi-cellular organisms control complex physiological processes ranging from cellular differentiation to development to aging. Traditional methods to investigate gene expression relationships rely on using bulk, pooled-population assays (e.g. RNA-sequencing and RT-PCR) to compare gene expression levels in hypo- or hyper-morphic mutant animals (e.g. gain-of-function or knockout). This approach is limited, especially in complex gene networks, as these genetic mutations may affect the expressions of related genes in unforseen ways. In contrast, we developed a microfluidic-based pipeline to discover gene relationships in a single genetic background. The microfluidic device provides efficient reagent exchange and the ability to track individua

SUBMITTER: Wan J 

PROVIDER: S-EPMC8146400 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature