Dynamics of cattle sperm sncRNAs during maturation, from testis to ejaculated sperm.

ABSTRACT:

Background

During epididymal transit, spermatozoa go through several functional maturation steps, resulting from interactions with epididymal secretomes specific to each region. In particular, the sperm membrane is under constant remodeling, with sequential attachment and shedding of various molecules provided by the epididymal lumen fluid and epididymosomes, which also deliver sncRNA cargo to sperm. As a result, the payload of sperm sncRNAs changes during the transit from the epididymis caput to the cauda. This work was designed to study the dynamics of cattle sperm sncRNAs from spermatogenesis to final maturation.

Results

Comprehensive catalogues of sperm sncRNAs were obtained from testicular parenchyma, epididymal caput, corpus and cauda, as well as ejaculated semen from

SUBMITTER: Sellem E 

PROVIDER: S-EPMC8146655 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature