MYD88^L265P Detection in IgM Monoclonal Gammopathies: Methodological Considerations for Routine Implementation.

ABSTRACT: In IgM monoclonal gammopathies MYD88^L265P is a prognostic and predictive biomarker of therapy response. MYD88^L265P detection is mainly performed by allele-specific quantitative PCR (ASqPCR), however recently, droplet digital PCR (ddPCR) has been proved to be suitable for MYD88^L265P screening and minimal residual disease monitoring (MRD). This study compared ASqPCR and ddPCR to define the most sensitive method for MYD88^L265P detection in bone marrow (BM), peripheral blood (PB) sorted or unsorted CD19+ cells, and in plasma cell-free DNA (cfDNA). Overall, the analysis showed a good concordance rate (74%) between the two methods, especially in BM samples, while discordances (26%) were mostly in favor of ddPCR (ddPCR+ vs. ASqPCR-) and were particularl

SUBMITTER: Ferrante M 

PROVIDER: S-EPMC8146978 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature