Influence of Culture Substrates on Morphology and Function of Pulmonary Alveolar Cells In Vitro.

ABSTRACT: Cell's microenvironment has been shown to exert influence on cell behavior. In particular, matrix-cell interactions strongly impact cell morphology and function. The purpose of this study was to analyze the influence of different culture substrate materials on phenotype and functional properties of lung epithelial adenocarcinoma (A549) cells. A549 cells were seeded onto two different biocompatible, commercially available substrates: a polyester coverslip (Thermanox™ Coverslips), that was used as cell culture plate control, and a polydimethylsiloxane membrane (PDMS, Elastosil® Film) investigated in this study as alternative material for A549 cells culture. The two substrates influenced cell morphology and the actin cytoskeleton organization. Further, the Yes-associated protein (YAP) and its

SUBMITTER: Campiglio CE 

PROVIDER: S-EPMC8147120 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature