A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs.

ABSTRACT: Circulating miRNAs are ideal diagnostics and prognostics biomarkers in cancer since altered levels of specific miRNAs have been associated to development/progression of several cancers. Physical activity is a recognized preventive strategy against several cancers, but it may also modify the baseline levels of cancer-associated miRNAs and, hence, may act as a confounding pre-analytical variable. This study aimed at understanding whether physical activity-dependent changes in cancer-associated circulating miRNAs profile could act as a confounding variable. A panel comprising 179 miRNAs was assayed in plasma from 20 highly trained and 10 sedentary men. RT-qPCR data were analyzed with the 2^-2ΔΔCT methods and normalized on hsa-miR-320d, as determined by bioinformatics analysis. miRNA

SUBMITTER: Faraldi M 

PROVIDER: S-EPMC8147229 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature