Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles.

ABSTRACT: To understand the factors that control the formation of the biomolecular corona, a systematic study of the adsorption of several miRNAs shown to be important in prostate cancer on amine-functionalized mesoporous silica nanoparticles (MSN-NH2) has been performed. Process parameters including miRNA type, nanoparticle concentration, incubation temperature and incubation time were investigated, as well as the potential competition for adsorption between different miRNA molecules. The influence of proteins and particle PEGylation on miRNA adsorption were also explored. We found that low particle concentrations and physiological temperature both led to increased miRNA adsorption. Adsorption of miRNA was also higher when proteins were present in the same solution; reducing or preventing protein a

SUBMITTER: Vidaurre-Agut C 

PROVIDER: S-EPMC8147382 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature