Phenotypic and Functional Heterogeneity of Low-Density and High-Density Human Lung Macrophages.

ABSTRACT: Pulmonary macrophages are a highly heterogeneous cell population distributed in different lung compartments. We separated two subpopulations of macrophages from human lung parenchyma according to flotation over density gradients. Two-thirds 65.4% of the lung macrophages have a density between 1.065 and 1.078 (high-density macrophages: HDMs), and the remaining one-third (34.6) had a density between 1.039 and 1.052 (low-density macrophages: LDMs). LDMs had a larger area (691 vs. 462 μm2) and cell perimeter (94 vs. 77 μm) compared to HDMs. A significantly higher percentage of HDMs expressed CD40, CD45, and CD86 compared to LDMs. In contrast, a higher percentage of LDMs expressed the activation markers CD63 and CD64. The release of TNF-α, IL-6, IL-10 and IL-12 induced by lipopolysaccharide (LP

SUBMITTER: Balestrieri B 

PROVIDER: S-EPMC8147777 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature