Cationic zinc is required for factor XII recruitment and activation by stimulated platelets and for thrombus formation in vivo.

ABSTRACT:

Background

Although divalent zinc (Zn²⁺ ) is known to bind factor (F)XII and affect its sensitivity to autoactivation, little is known about the role of Zn²⁺ in the binding of FXII to platelets, where FXII activation is thought to occur in vivo, and the function of Zn²⁺ during thrombus formation following vascular injury remains poorly understood.

Objectives

To evaluate the role of Zn²⁺ in platelet-dependent FXIIa generation.

Methods

FXII binding to platelets and FXII activation by stimulated platelets were assessed using flow cytometry and a platelet-dependent thrombin generation assay. The mouse cremaster laser injury model was used to evaluate the impact of Zn²⁺ chelation on thrombus formation in vivo.

Resul

SUBMITTER: Chaudhry SA 

PROVIDER: S-EPMC8147875 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature