A robust photoluminescence screening assay identifies uracil-DNA glycosylase inhibitors against prostate cancer.

ABSTRACT: Many cancers have developed resistance to 5-FU, due to removal by the enzyme uracil-DNA glycosylase (UDG), a type of base excision repair enzyme (BER) that can excise uracil and 5-fluorouracil (5-FU) from DNA. However, the development of UDG inhibitor screening methods, especially for the rapid and efficient screening of natural product/natural product-like compounds, is still limited so far. We developed herein a robust time-resolved photoluminescence method for screening UDG inhibitors, which could significantly improve sensitivity over the screening method based on the conventional steady-state spectroscopy, reducing the substantial fluorescence background interference. As a proof-of-concept, two potential UDG inhibitors were identified from a database of natural products and approved d

SUBMITTER: Li G 

PROVIDER: S-EPMC8148385 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature