Hepcidin Gene Cloning and Expression Pattern in Turbot (Scophthalmus maximus) after Vibrio. anguillarum Infection.

ABSTRACT:

Background

Antimicrobial peptides play crucial roles in organisms as the first line of defense against invading pathogens.

Objective

To isolate the hepcidin (hepc1) gene from the liver of turbot (Scophthalmus maximus) challenged with Vibrio anguillarum (GenBank accession number: AM113708), characterize it, and assess its expression level in various tissues.

Materials and methods

The DNA sequence of hepcidin from S. maximus was determined from the total RNA extracted and reverse transcribed from this fish. The expression levels of tissue-specific hepcidin transcripts were determined using reverse-transcriptase polymerase chain reactions.

Results

Hepcidin levels increased in the livers, head kidneys and spleens of the fish. The transcr

SUBMITTER: Deng AF 

PROVIDER: S-EPMC8148637 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature