Identification of Subgenomic DNAs Associated with Wheat Dwarf Virus Infection in Iran.

ABSTRACT:

Background

Wheat dwarf virus (WDV) is a leafhopper-transmitted DNA virus which causes yellowing and stunting in wheat and barley fields leading to considerable crop loss around the world. Mainly, two host-specific forms of WDV have been characterized in wheat and barley (WDV-Wheat and WDV-Barley, respectively).

Objectives

This study was aimed to amplify, sequence and describe subgenomic DNAs (sgDNAs) associated with WDV infection among wheat and barley plants. The nucleotide sequence of sgDNAs were then compared to that of parental genomic DNAs (gDNAs) and the differences were shown.

Materials and methods

A total of 65 symptomatic plants were surveyed for WDV infection using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and polymerase c

SUBMITTER: Ghodoum Parizipour MH 

PROVIDER: S-EPMC8148644 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature