Histological and transcriptional characterization of the pancreatic acinar tissue in type 1 diabetes.

ABSTRACT:

Introduction

Despite a reduced function and volume of the exocrine pancreas in type 1 diabetes, the acinar cells remain understudied in type 1 diabetes research. The hypothesis of this study is that the acinar tissue is altered in subjects with type 1 diabetes compared with subjects without diabetes.

Research design and methods

The cell density, expression of digestive enzymes, and transcriptome of acinar tissue at varying distances from islets were analyzed using histology, immunostaining, and AmpliSeq RNA sequencing of laser capture microdissected tissue. Pancreases examined were from organ donors with or without type 1 diabetes.

Results

We demonstrate preserved acinar nuclei density and find no support of acinar atrophy in type 1 diabetes. Staining for digestive en

SUBMITTER: Granlund L 

PROVIDER: S-EPMC8149357 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature