Single channel properties of mitochondrial large conductance potassium channel formed by BK-VEDEC splice variant.

ABSTRACT: The activation of mitochondrial large conductance calcium-activated potassium (mitoBK_Ca) channels increases cell survival during ischemia/reperfusion injury of cardiac cells. The basic biophysical and pharmacological properties of mitoBK_Ca correspond to the properties of the BK_Ca channels from the plasma membrane. It has been suggested that the VEDEC splice variant of the KCNMA1 gene product encoding plasma membrane BK_Ca is targeted toward mitochondria. However there has been no direct evidence that this protein forms a functional channel in mitochondria. In our study, we used HEK293T cells to express the VEDEC splice variant and observed channel activity in mitochondria using the mitoplast patch-clamp technique. For the first time, we found that

SUBMITTER: Galecka S 

PROVIDER: S-EPMC8149700 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature