Nasal Extracts from Patients with Alzheimer's Disease Induce Tau Aggregates in a Cellular Model of Tau Propagation.

ABSTRACT:

Background

Emerging evidence indicates that the misfolded tau protein can propagate aggregates between cells in a prion-like manner. This prion activity has been typically studied in brain extracts of patients with Alzheimer's disease (AD), but not in the olfactory region that can be a potential biomarker in AD.

Objective

To investigate the prion seeding activity of tau in nasal mucosa tissues using a cell culture model of tau propagation.

Methods

Brain and nasal mucosa homogenates were added to HEK293T cells expressing three repeat or four-repeat domains of tau with the L266V, V337M (3RD^*VM) and P301L and V377M mutations (4RD^*LM) fused to the enhanced green fluorescence protein (EGFP) respectively. We also measured the level of phosphorylated ta

SUBMITTER: Pahrudin Arrozi A 

PROVIDER: S-EPMC8150252 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature