Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing.

ABSTRACT: Coccidioides immitis and C. posadasii are dimorphic fungi that transform from mycelia with internal arthroconidia in the soil to a tissue form known as a spherule in mammals. This process can be recapitulated in vitro by increasing the temperature, CO₂ and changing other culture conditions. In this study, we have analyzed changes in gene expression in mycelia and young and mature spherules. Genes that were highly upregulated in young spherules include a spherule surface protein and iron and copper membrane transporters. Genes that are unique to Coccidioides spp. are also overrepresented in this group, suggesting that they may be important for spherule differentiation. Enriched GO terms in young spherule upregulated genes include oxidation-reducti

SUBMITTER: Carlin AF 

PROVIDER: S-EPMC8150946 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature