Bacteria Single-Cell and Photosensitizer Interaction Revealed by Quantitative Phase Imaging.

ABSTRACT: Quantifying changes in bacteria cells in the presence of antibacterial treatment is one of the main challenges facing contemporary medicine; it is a challenge that is relevant for tackling issues pertaining to bacterial biofilm formation that substantially decreases susceptibility to biocidal agents. Three-dimensional label-free imaging and quantitative analysis of bacteria-photosensitizer interactions, crucial for antimicrobial photodynamic therapy, is still limited due to the use of conventional imaging techniques. We present a new method for investigating the alterations in living cells and quantitatively analyzing the process of bacteria photodynamic inactivation. Digital holographic tomography (DHT) was used for in situ examination of the response of Escherichia coli and Sta

SUBMITTER: Buzalewicz I 

PROVIDER: S-EPMC8151141 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature