CD47 Potentiates Inflammatory Response in Systemic Lupus Erythematosus.

ABSTRACT: To investigate the role of CD47 in inflammatory responses in systemic lupus erythematosus (SLE). Expression of CD47 and signal regulatory protein alpha (SIRPα) by peripheral blood mononuclear cells (PBMCs) and changes in CD47 expression after exposure to SLE serum, healthy control (HC) serum, recombinant interferon (IFN)-α, or tumor necrosis factor (TNF)-α were examined. Human monocytes and THP1 cells were incubated with lipopolysaccharide (LPS), an anti-CD47 antibody, or both. TNF-α production was examined. Sera from SLE patients and HCs were screened to detect autoantibodies specific for CD47. Twenty-five SLE patients and sixteen HCs were enrolled. CD47 expression by monocytes from SLE patients was higher than those from HCs (mean fluorescence intensity ± SD: 815.9 ± 269.4 vs. 511.5 ± 19

SUBMITTER: Park JK 

PROVIDER: S-EPMC8151692 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature