Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics.

ABSTRACT: Previously, DNA microarrays analysis showed that, in co-culture with Bacillus subtilis, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of Aspergillus niger is downregulated. Based on phylogenetic and synteny analyses, we show here that this gene cluster, NRRL3_00036-NRRL3_00042, comprises genes predicted to encode a nonribosomal peptides synthetase, a FAD-binding domain-containing protein, an uncharacterized protein, a transporter, a cytochrome P450 protein, a NAD(P)-binding domain-containing protein and a transcription factor. We overexpressed the in-cluster transcription factor gene NRRL3_00042. The overexpression strain, NRRL3_00042^OE, displays reduced growth rate and production of a yellow pigment, which by mass spect

SUBMITTER: Evdokias G 

PROVIDER: S-EPMC8151901 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature