CesL Regulates Type III Secretion Substrate Specificity of the Enteropathogenic E. coli Injectisome.

ABSTRACT: The type III secretion system (T3SS) is a complex molecular device used by several pathogenic bacteria to translocate effector proteins directly into eukaryotic host cells. One remarkable feature of the T3SS is its ability to secrete different categories of proteins in a hierarchical manner, to ensure proper assembly and timely delivery of effectors into target cells. In enteropathogenic Escherichia coli, the substrate specificity switch from translocator to effector secretion is regulated by a gatekeeper complex composed of SepL, SepD, and CesL proteins. Here, we report a characterization of the CesL protein using biochemical and genetic approaches. We investigated discrepancies in the phenotype among different cesL deletion mutants and showed that CesL is indeed essential f

SUBMITTER: Diaz-Guerrero M 

PROVIDER: S-EPMC8152094 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature